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      • Powered By
        Strikingly
        • James DiCarlo, M.D., Ph.D.

          Synthetic Biologist and Physician

        • Hello!

          My name is James

          I have always been interested in living things.

           

          As a physician-scientist I aim to find methods to responsibly modify those living things to best suit the needs of humanity.

        • Publications

          Please check out my publications. I think they are great and I am not biased.

          Gene therapy and genome surgery in the retina​

          DiCarlo, J.E., Mahajan V.B., Tsang S.H.

           

          Journal of Clinical Investigation 2018

          High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR–Cas9 in yeast

          Guo, X., Chavez, A., Tung, A., Chan, Y., Cecchi, R., Garnier, S.L., Kaas, C., Kelsic, E., Schubert, M., DiCarlo, J.E., Collins, J.J., Church, G.M.

           

          Nature Biotechnology 2018

          A CRISPR–Cas9-based gene drive platform for genetic interaction analysis in Candida albicans

          Shapiro R.S., Chavez A., Porter C.B., Hamblin M., Kaas C.S., DiCarlo J.E., Zeng G., Xu X., Alexey V. Revtovich A.V., Kirienko N.V., Wang Y., Church G.M., Collins J.J.

           

          Nature Microbiology 2017

          Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

          Cabral, T, Toral, M.A., Velez, G., DiCarlo, J.E., Gore A.M., Mahajan, M, Tsang, S.H., Bassuk, A.G, Mahajan, V.B.

           

          Journal of Visualized Experiments 2017

          Design of a synthetic yeast genome

          Sarah M Richardson, Leslie A Mitchell, Giovanni Stracquadanio, Kun Yang, Jessica S Dymond, James E DiCarlo, Dongwon Lee, Cheng Lai Victor Huang, Srinivasan Chandrasegaran, Yizhi Cai, Jef D Boeke, Joel S Bader

           

          Science 2017

          CRISPR-Cas Genome Surgery in Ophthalmology

          James E. DiCarlo, Jesse D. Sengillo, Sally Justus, Thiago Cabral, Stephen H. Tsang, Vinit B. Mahajan

           

          Translational Vision Science & Technology 2017

          CRISPR applications in ophthalmologic genome surgery

          Cabral T*, DiCarlo JE*, Justus S, Sengillo JD, Xu Y, Tsang SH

           

          Current Opinion in Ophthalmology 2017

          Safeguarding CRISPR-Cas9 gene drives in yeast

          DiCarlo JE, Chavez A, Dietz SL, Esvelt KM, Church GM

           

          Nature Biotechnology 2015

          Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

          DiCarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.

           

          Nucleic acids research 2013

          Yeast Oligo-Mediated Genome Engineering (YOGE)

          DiCarlo JE, Conley AJ, Penttilä M, Jäntti J, Wang HH, Church GM.

           

          ACS Synthetic Biology 2013

          RNA-guided human genome engineering via Cas9

          Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM.

           

          Science 2013

          Viral Vectors, Engineered Cells and the CRISPR Revolution

          DiCarlo, J.E., Deeconda, A, Tsang S.H

           

          Book: Precision Medicine, CRISPR, and Genome Engineering 2017

          Total Synthesis of a Functional Designer Eukaryotic Chromosome

          Annaluru N, Muller H, Mitchell LA, Ramalingam S, Stracquadanio G, Richardson SM, Dymond JS, Kuang Z, Scheifele LZ, Cooper EM, Cai Y, Zeller K, Agmon N, Han JS, Hadjithomas M, Tullman J, Caravelli K, Cirelli K, Guo Z, London V, Yeluru A, Murugan S, Kandavelou K, Agier N, Fischer G, Yang K, Martin JA, Bilgel M, Bohutski P, Boulier KM, Capaldo BJ, Chang J, Charoen K, Choi WJ, Deng P, DiCarlo JE, Doong J, Dunn J, Feinberg JI, Fernandez C, Floria CE, Gladowski D, Hadidi P, Ishizuka I, Jabbari J, Lau CY, Lee PA, Li S, Lin D, Linder ME, Ling J, Liu J, Liu J, London M, Ma H, Mao J, McDade JE, McMillan A, Moore AM, Oh WC, Ouyang Y, Patel R, Paul M, Paulsen LC, Qiu J, Rhee A, Rubashkin MG, Soh IY, Sotuyo NE, Srinivas V, Suarez A, Wong A, Wong R, Xie WR, Xu Y, Yu AT, Koszul R, Bader JS, Boeke JD, Chandrasegaran S.

           

          Science (2014)

        • Training

          Brigham and Women's Hospital (2019-Present)

          Resident Physician

          Department of Pathology

           

          Columbia University College of Physicians & Surgeons (2015-2019)

          M.D.

          M.D. Program

          Boston University (2010-2015)

          Ph.D., M.S. Biomedical Engineering

          Laboratory of George Church

          (Harvard Medical School)

           

          Thesis: "Engineering Yeast Genomes and Populations"

           

          Johns Hopkins University (2006-2010)

          B.S. Chemical and Biomolecular Engineering

          Laboratory of Jef Boeke

          (New York University)

           

          Projects - Synthetic Yeast, Genome Evolution

        •  

          My Reef tank

           

          I am very proud of my little reef aquarium which I started in December 2019. It is a hobby I have been involved with for almost two decades.

           

          Tank Specifications:

           

          Tank: Innovative Marine 10 NUVO Fusion

          Light: A360X Tuna Blue

          Circulation: MightyJet Desktop AIO DC Return Pump (326 GPH) - Innovative Marine

           

           

          Livestock:

          Acanthastrea pachysepta

          Acanthastrea lordhowensis

          Montipora setosa

          Montipora capricornis

          Montipora danae "Superman"

          Ricordea yuma

          Sarcophyton sp.

           

           

          Zoanthids - ASD Angel Tears, Alpha & Omega, King Midas, Halle Berry

           

           

           

        • Contact

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